A physiological super-resolution recording of norepinephrine release from mouse PNS-organs or clinical biopsies in situ

Date:2023-04-18

 

Time: 14:00-15:30 on Tue.,April.18 2023

Venue:E109, Biomedicine Hall

Speaker: Dr.Zhuan Zhou

Host: Dr.Song-Hai Shi

Title:  A physiological super-resolution recording of norepinephrine release from mouse PNS-organs or clinical biopsies in situ 

 

Abstract:

Norepinephrine (NE) released from the peripheral nervous system (PNS) is responsible for diverse peripheral functions; they are potential therapeutic targets in many diseases. However, the spatiotemporal resolution of PNS-NE distribution and release dynamics and its mechanisms remain poorly characterized, due to technical limitations. Here, we develop and validate a patch-clamp like method to record sympathetic NE release in any fresh peripheral organ slices with ElectroChemistry (SEC) via a micro carbon fiber electrode (1-7 μm). SEC directly monitors PNS-NE release in slices from all tested peripheral organs (heart, spleen, fat, et al.) with a combined super spatiotemporal resolution-sensitivity at the micrometer, millisecond, and nanomole (μm-ms-nM) level. Using SEC, we recorded an elevated (180 ± 20 %) subsecond NE release in heart in lipopolysaccharide-induced inflammation. SEC also demonstrated the precise cardiac PNS-NE release and reuptake alterations in a mouse model of heart failure (HF), and solved a decades-long issue about the unknown changes of NE kinetics in HF. Importantly, the SEC method is also capable of phenotyping NE release in tiny human biopsies (≥1mm3). Thus, the SEC method provides reliable, broadly applicable tools to investigate the spatiotemporal landscape of NE release in peripheral organs under pathophysiological conditions in animals and humans.

 

Biography:

Dr. Zhuan Zhou is a professor in College of Future Technology, Peking University, China. His research interest is on dopamine/monoamine secretion in physiology and diseases, mechanisms of exocytosis and endocytosis, PD-impaired neural circuits using [Ca]i imaging and DA recordings in awaking rodents, mechanisms of monoamine release from PNS nerves in slices & in vivo, design & developing new methods/tools for physiological recordings. He has been awarded the National Outstanding Young Scholar. He has published over 100 scientific journal papers on peer-reviewed journals including Nature Neuroscience, Neuron, Proc Natl Acad Sci U S A., Nature Communications etc. He was the vice president of Biophysical Society of China (2006-2010) and the chair of neurobiophysical committee (2002-2006, 2006-2010).