Time: 14:00-15:30 on Tue.,April.18 2023

Venue：E109, Biomedicine Hall

Speaker: Dr.Zhuan Zhou

Host: Dr.Song-Hai Shi

Title:  A physiological super-resolution recording of norepinephrine release from mouse PNS-organs or clinical biopsies in situ 

Abstract：

Norepinephrine (NE) released from the peripheral nervous system (PNS) is responsible for diverse peripheral functions; they are potential therapeutic targets in many diseases. However, the spatiotemporal resolution of PNS-NE distribution and release dynamics and its mechanisms remain poorly characterized, due to technical limitations. Here, we develop and validate a patch-clamp like method to record sympathetic NE release in any fresh peripheral organ slices with ElectroChemistry (SEC) via a micro carbon fiber electrode (1-7 μm). SEC directly monitors PNS-NE release in slices from all tested peripheral organs (heart, spleen, fat, et al.) with a combined super spatiotemporal resolution-sensitivity at the micrometer, millisecond, and nanomole (μm-ms-nM) level. Using SEC, we recorded an elevated (180 ± 20 %) subsecond NE release in heart in lipopolysaccharide-induced inflammation. SEC also demonstrated the precise cardiac PNS-NE release and reuptake alterations in a mouse model of heart failure (HF), and solved a decades-long issue about the unknown changes of NE kinetics in HF. Importantly, the SEC method is also capable of phenotyping NE release in tiny human biopsies (≥1mm3). Thus, the SEC method provides reliable, broadly applicable tools to investigate the spatiotemporal landscape of NE release in peripheral organs under pathophysiological conditions in animals and humans.

Biography：

Dr. Zhuan Zhou is a professor in College of Future Technology, Peking University, China. His research interest is on dopamine/monoamine secretion in physiology and diseases, mechanisms of exocytosis and endocytosis, PD-impaired neural circuits using [Ca]i imaging and DA recordings in awaking rodents, mechanisms of monoamine release from PNS nerves in slices & in vivo, design & developing new methods/tools for physiological recordings. He has been awarded the National Outstanding Young Scholar. He has published over 100 scientific journal papers on peer-reviewed journals including Nature Neuroscience, Neuron, Proc Natl Acad Sci U S A., Nature Communications etc. He was the vice president of Biophysical Society of China (2006-2010) and the chair of neurobiophysical committee (2002-2006, 2006-2010).