Li, Yinqing

PI,IDG/McGovern Institute, Tsinghua University
PI,School of Pharmaceutical Sciences, Tsinghua University

[Research Focus]

Yinqing Li has a longstanding interest in developing technologies for better diagnosis and management of complex diseases. In particular, his research focuses on methods for identifying key molecular and cell players in the development of diseases and methods for gene and cell editing. Specific approaches include: single cell multi-omics of epigenetics, transcriptome, and signaling pathway profiling, discovery of novel genome and epigenome editing tools, and statistical inference and machine learning algorithms for high throughput data mining and processing.

[Education & Experience]

2018-present   PI, IDG/McGovern Institute, School of Pharmaceutical Sciences, Tsinghua University
2016-2018       Postdoctoral research in the Broad Institute of Harvard and MIT and Stanley Center for Psychiatric Research
2008-2016       MS and PhD in Electrical Engineering and Computer Science from MIT
2004-2008       B.S. in Microelectronics from Fudan University, China

[Selected Publications]

  • Li Y*, Weiss R., A Modular Approach to Building Complex Synthetic Circuits. Methods in Molecular Biology. 2017. pp. 231–248, DOI:10.1007/978-1-4939-7223-4_17.
  • Yan WX, Mirzazadeh R, Garnerone S, Scott D, Schneider MW, Kallas T, Custodio J, Wernersson E, Li Y, Gao L, Federova Y, Zetsche B, Zhang F, Bienko M, Crosetto N., BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks. NATURE COMMUNICATIONS. 2017, 8:15058.
  • Habib N*, Li Y*, Heidenreich M, Swiech L, Avraham-Davidi I, Trombetta JJ, Hession C, Zhang F, Regev A. Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons. SCIENCE. 2016, 353(6302):925-8.
  • Yamano T, Nishimasu H, Zetsche B, Hirano H, Slaymaker IM, Li Y, Fedorova I, Nakane T, Makarova KS, Koonin EV, Ishitani R, Zhang F, Nureki O. Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA. CELL. 2016, 165(4):949-62.
  • Nishimasu H, Cong L, Yan W, Ran F, Bernd Z, Li Y, Kurabayashi A, Ishitani R, Zhang F, Nureki O. Crystal structure of Staphylococcus aureus Cas9. CELL. 2015, 162(5):1113-26.
  • Davidsohn N, Beal J, Kiani S, Adler A, Yaman F, Li Y, Xie Z, Weiss R. Accurate predictions of genetic circuit behavior from part characterization and modular composition. ACS SYNTHETIC BIOLOGY. 2015, 4(6):673-81.
  • Li Y*, Jiang Y*, Chen H*, Liao W, Li Z, Weiss R, Xie Z. Modular construction of mammalian gene circuits using TALE transcriptional repressors. NATURE CHEMICALBIOLOGY. 2015, 11(3):207-13.
  • Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. NATURE BIOTECHNOLOGY. 2015, 33(1):102-6.
  • Duportet X, Wroblewska L, Guye P, Li Y, Eyquem J, Rieders J, Rimchala T, Batt G, Weiss R. A platform for rapid prototyping of synthetic gene networks in mammalian cells. NUCLEIC ACIDS RESEARCH. 2014, 42(21):13440-51.
  • Busskamp V, Lewis NE, Guye P, Ng AH, Shipman SL, Byrne SM, Sanjana NE, Murn J, Li Y, Li S, Stadler M, Weiss R, Church GM. Rapid neurogenesis through transcriptional activation in human stem cells. MOLECULAR SYSTEMS BIOLOGY. 2014, 10:760.
  • Kiani S, Beal J, Ebrahimkhani MR, Huh J, Hall RN, Xie Z, Li Y, Weiss R. CRISPR transcriptional repression devices and layered circuits in mammalian cells. NATURE METHODS. 2014, 11(7):723-6.
  • Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini LA, Bao G, Zhang F. DNA targeting specificity of RNA-guided Cas9 nucleases. NATURE BIOTECHNOLOGY. 2013, 31(9):827-32.
  • Guye P*, Li Y*, Wroblewska L, Duportet X, Weiss R. Rapid, modular and reliable construction of complex mammalian gene circuits. NUCLEIC ACIDS RESEARCH. 2013, 41(16):e156.